Regulatory

Part:BBa_K3905000:Design

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-04)


gen1 517-5p Toehold Switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to ensure that the toehold switch's sequence did not bind to the linker sequence deforming the structure, that the start codon was not exposed in the 'off' state and that the RBS held the correct formation in the 'on' state.


Source

No source. Designed using NUPACK. Synthetic.

References